Scientific Publishers | mycological-techniques

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Blurb

Mycological Techniques: Identification of Mycotoxigenic Fungi and Mycotoxins” is a manual designed to aid the guidelines and techniques applied in mycological laboratory and in the other allied fields. This handbook is based on research conducted by many renowned scientists on fungi and related mycotoxins, and the practical approach to the isolation and identification of toxigenic strains of fungi as well as their related fungal toxins, called as Mycotoxins, commonly met on stored food and other materials. Students hopefully will find the information on important fungi particularly related to storage and field conditions and secondary metabolites produced during the growth of fungi on food and other substrates.Reports of many researchers, scientists, and book from all over globe indicate direct relation between the incidence of mycotoxigenic fungi, extent of mycotoxin contamination and their prevalence revealed their relation to some of the human ailments. Most of the mycotoxins mainly aflatoxins, ochratoxins A and fumonisins are posing serious health hazards in Asian countries. In the context of Indian climatic conditions, need of assessing and preparation of a comprehensive account related to consumption of contaminated food and feed is essential in order to highlight the problems and their health hazards due to mycotoxins. Present attempt is made to provide recent developments in the subject so that researchers interested may get clear understanding of the problems.

Preface

1. Introduction:

Laboratory Safety; Sterilization, Sterile Distilled Water.

2. Culture Media:

Important Ingredients in Culture Media, Growth Media, Composition of Important Media.

3. Mycological Evaluation / Isolation Study Techniques:

Methods of Seed Examination, Standard Methods for Mycological Evaluation of Contaminated Samples; Deep Freezing Method, Direct Microscopic Examination: Slide Culture Method, Application of Stain, Advantages of Slide Culture; Serial Dilution Agar Plate Technique, Single Spore Technique, Pour Plate Technique; Technique to Study Litter Decomposing and Leaf Spot Pathogenic Fungi

4. Identification of Fungus Grown in Culture:

Microscopic Techniques of Identification: Micrometery, Slide Culture Technique, Haemocytometer, Camera Lucida; Cryopreservation; Koch’s Postulates.

5. Morpho-Taxonomic Description of Mycotoxic Fungi:

Aspergillus : Section: Aspergillus, Section: Fumigati, Section: Clavati, Section: Nidulantes, Section: Versicolors, Section: Usti, Section: Terrei, and Section: Wentii ; Penicillium, Fusarium, Alternaria, Trichoderma, Trichothecium, Phoma, Phomopsis, Stachybotrys, Syncephalastrum, Chaetomium, Curvularia, Colletotrichum, and Cladosporium.

6. Mycotoxins:

Aflatoxins, Kojic Acid, Fumonisins, Patulin, Ochratoxin, Stergmatocystin, Ergot, Esterogenic mycotoxins (Fusarial toxins): Trichothecenes.

7. Methods of Mycotoxin Analysis:

Screening Test, Qualitative Methods: Determination of mycotoxins by Thin Layer Chromatography; Estimation of Mycotoxins: Aflatoxin, Ochratoxin, Zearalenone; Determination of Aflatoxins by Pressure Mini Column Method; Units of Concentration.

8.References

9. Glossary of Mycological Terms